Super Resolution Imaging and Structure Measurements of Chromatin and of Supercoiled DNA Plasmids

by E. Benjamin, I. Michaelovich

at Quantum optics seminar

Thu, 10 May 2018, 15:30
Physics building (#54) room 207

Abstract

The organisation of the nucleic material in eukaryotic cells the chromatin affects many cellularprocesses Though chromatin has been subject of research for almost 150 years it s exact structure in the sub chromosome scale is still unknown We present a study of chromatin s structure inside the nucleus using a combination of scanning fluorescent correlation spectroscopy sFCS and super resolution time gated stimulated emission depletion microscopy gSTED Our preliminary results shows evidence of Gaussian shaped sub structures on the scale of 300 400 nm a behaviour typical of a beads on a spring system Furthermore we used the combined sFCS gSTED approach to measure the structure of superhelical DNA in solution We compare the experimental measurements to theoretical models and computer simulations that take into account DNA twist and bending rigidity electrostatic interactions and DNA configurational entropy

Created on 06-05-2018 by Bar Lev, Yevgeny (ybarlev)
Updaded on 06-05-2018 by Bar Lev, Yevgeny (ybarlev)