Basic and clinical science applications using Quantitative Phase Imaging

by Gabriel Popescu

at Quantum optics seminar

Thu, 15 Nov 2018, 15:30
Physics building (#54) room 207

Abstract

Light scattering limits the quality of optical imaging of unlabeled specimens: too little scattering and the sample is transparent exhibiting low contrast and too much scattering washes the structure information altogether As a result current instruments target specifically either the thin low scattering specimens or the optically thick multiply scattering samples In 2011 we developed spatial light interference microscopy SLIM as a high sensitivity high resolution quantitative phase imaging method which open new applications for studying structure and dynamics Color SLIM cSLIM is a recent development that allows the phase imaging of stained tissue slices Using specimens prepared under the standard protocols in pathology cSLIM yields simultaneously the typical image that the pathologist is accustomed to e g H amp E immunochemical stains etc and a quantitative phase image which provides new information currently not available in bright field images e g collagen fiber orientation However SLIM works best for thin specimens such as single cell layers and tissue slices To expand this type of imaging to thick multiply scattering media we developed gradient light interference microcopy GLIM GLIM exploits the principle of low coherence interferometry to extract phase information which in turn yields strong intrinsic contrast of transparent samples such as single cells Because it combines multiple intensity images that correspond to controlled phase shifts between two interfering waves GLIM is capable of suppressing the incoherent background due to multiple scattering We demonstrate the use of GLIM to image various samples including standard micron size beads single cells cell populations thick bovine embryos and live brain slices GLIM operates as an add on to a conventional microscope and overlays seamlessly with the existing channels e g fluorescence

Created on 07-11-2018 by Bar Lev, Yevgeny (ybarlev)
Updaded on 07-11-2018 by Bar Lev, Yevgeny (ybarlev)