Measuring cellular state transitions across scales

Obtaining a holistic mechanistic understanding of biological processes relies on the ability to continuously measure the physiological states of cells and molecular machines through time. We are leveraging live cell imaging and computation to systematically measure cellular state transitions across scales. In my talk, I will present three projects. At the multicellular scale, we followed the process of collective calcium dynamics synchronization of endothelial monolayers in response to mechanical stimuli. At the single cell scale, we followed the process of primary myoblasts undergoing differentiation and fusion to form multinucleated muscle fibers ex vivo. At the molecular scale, we are currently developing a method to enable systematic structural characterization of the dynamic evolution of biomolecular complexes from single molecule localization microscopy.