Gas-Phase Biomolecules: Structural Motifs Revealed by Ionization-Loss Stimulated Raman Spectroscopy

by Mr. Afik Shachar

at Special seminar

Tue, 27 Apr 2021, 13:00


Student seminar
ZOOM link:

Exposing the dynamics, three-dimensional structures, and conformational landscapes of isolated biomolecules and the role played by their inter- and intra-molecular interactions with each other and with solvents attracts considerable interest. Coupling the results of the novel laser-based spectroscopic technique of ionization-loss stimulated Raman spectroscopy (ILSRS) and quantum calculations allows retrieving the vibrational signatures of molecules in a broad frequency range. This approach provided the structural motifs and a molecular-level understanding of a model fluorinated neurotransmitter and its hydrated cluster. I further demonstrate the universality of this approach by applying it to non-volatile biomolecules, which required the development of a compact laser desorption (LD) source for molecular beam (MB) generation. The combination of spectroscopic measurements with Monte-Carlo simulations enabled source optimization and characterization. This study significantly enhanced our understanding of the physical principles governing the structures of the explored species and allowed the first combination of LD/ILSRS. The approach developed here provides access to vibrational signatures of isolated non-volatile molecules by tabletop laser sources and will provide new insight into biomolecules.

Created on 21-04-2021 by Folman, Ron (folman)
Updaded on 21-04-2021 by Folman, Ron (folman)