Scanning Probe Microscope-based approaches to nano-biochips
Levi A. Gheber
Department of Biotechnology Engineering
Ben-Gurion University of the Negev, Beer-Sheva, Israel
The maturation of arrayed biosensors (mainly DNA micro-arrays,
known as "DNA chips") has demonstrated the power of the parallel
approach to sensing in general and biosensing in particular. The
ability to ask thousand of questions and obtain simultaneous answers
enables high-throughput screening of potential drugs, diagnosis of
genetically derived disorders and basic genome research. The promise of
the biochip, though, goes much further and farther: online monitoring
of drinking water and food quality, point-of-care diagnosis,
bio-defense and other futuristic applications. To reach there, though,
there is a need to almost completely transform the existing technology,
which at present is highly localized to large research laboratories,
big pharmaceutical companies or advanced medical centers.
Several aspects must be considered simultaneously (a step-by-step
improvement of existing technology is likely to fail): the "spot" size
needs to be reduced by several orders of magnitude, reporting and
reading systems must be integrated, sample preparation and handling
subsystems should also be integrated, the devices need to be
increasingly autonomous and have communication abilities, label-free
detection methods need to be developed, to enable on-line operation.
We will analyze the parameters that prevent portability and discuss
some approaches, mostly based on Scanning Probe Microscopy (SPM) that
may provide some answers to these challenges. Among the topics covered,
protein printing, enzyme nanolithography, polymer optics, MIP based
arrays and wettability patterning.